| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Ethan D (9)
|
MDGBSA |
41.32 |
42.11 |
0.79 |
| 2 |
Zhang, Ryan D (10)
|
MDEST |
41.65 |
42.62 |
0.97 |
| 3 |
Greenwald, Harrison A (10)
|
MDEST |
42.12 |
42.96 |
0.84 |
| 4 |
Liang, Kai H (10)
|
MDEST |
43.04 |
45.14 |
2.10 |
| 5 |
Yue, Kevin (8)
|
MDYCM |
45.25 |
45.47 |
0.22 |
| 6 |
Chen, Andrew L (9)
|
MDRAC |
52.49 |
45.69 |
-6.80 |
| 7 |
Yang, Arthur Z (10)
|
MDYCM |
43.44 |
45.93 |
2.49 |
| 8 |
Celtnieks, Eli (9)
|
MDMSC |
43.80 |
46.19 |
2.39 |
| 9 |
Erb, Gabriel B (10)
|
MDMAC |
43.82 |
46.26 |
2.44 |
| 10 |
Ddungu, Hashir I (10)
|
MDEST |
43.34 |
46.58 |
3.24 |
| 11 |
Schmeckpeper, Michael P (8)
|
MDRAC |
45.47 |
47.08 |
1.61 |
| 12 |
Kay, Tyler O (9)
|
MDEST |
51.11 |
47.92 |
-3.19 |
| 13 |
Li, Ryan (10)
|
MDEST |
52.00 |
48.28 |
-3.72 |
| 14 |
Zheng, Kevin W (8)
|
MDYCM |
47.93 |
49.69 |
1.76 |
| 15 |
Erb, Jeremiah S (8)
|
MDMAC |
49.96 |
50.88 |
0.92 |
|
Fox, Thomas (10)
|
MDMSC |
50.67 |
NS |
- |